Fig. 5

Pravastatin decreases insulin-induced AKT phosphorylation in C2C12 myotubes. a Representative Western blot of pAkt (Ser473), total Akt, and GAPDH in C2C12 myotubes treated with pravastatin (1, 10 or 50 µM) for 12 h. After pravastatin treatment, the myotubes were treated with 100 nM insulin for 10 min or left untreated. b The bar graphs depict the ratios of the intensities of the pAkt (Ser473) to Akt bands relative to the controls without insulin. Mean ± SE (three independent experiments). *p < 0.05 as determined by one-way analysis of variance (ANOVA) with LSD (Fisher´s least significance difference) post hoc test