Fig. 6

IKKβ in intestine is critical in the effects of antibiotic treatment on the phagocytic activity and peroxynitrite production of AMs, and IL-6 levels in plasma. a IKKβ of intestine is critical in the phagocytic activity of AMs and tryptophan, or dead L. plantarum feeding-induced phagocytic activity of AMs. Data are presented as mean ± SEM. b Antibiotic treatment significantly increases IL-6 plasma levels of Vil-Cre/Ikkβ^F/Δ mice. Antibiotics, antibiotic with Tryptophan, or antibiotic with dead L. plantarum did not change IL-6 plasma levels in Vil-Cre/Ikkβ^F/Δ as compared with the control group in Ikkβ^F/Δ mice. The mouse ELISA kit (eBioscience) was used for IL-6 assay. The blood was centrifuged at 1000×g, 4 °C for for 15 min and the serum was collected for use. Data are presented as mean ± SEM. c Peroxynitrite production of AMs of Vil-Cre/Ikkβ^F/Δ mice did not demonstrate a significant change as compared with that in Ikkβ^F/Δ mice. Antibiotics, antibiotic with Tryptophan, or antibiotic with dead L. plantarum did not change peroxynitrite production of AMs in Vil-Cre/Ikkβ^F/Δ as compared with that Ikkβ^F/Δ mice. The collected AMs were cultured with phenol red containing 25 μM of 1,2,3-dihydrorhodamine. The cells were stimulated with E. coli LPS. Peroxynitrite was measured every 15 min for 75 min using excitation and emission wavelengths of 485 and 530 nm, respectively. Data are presented as mean ± SEM; *P < 0.05, **P < 0.01