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Table 3 Primers for PCR amplification of TWNK exons and exon–intron boundaries

From: Perrault syndrome with neurological features in a compound heterozygote for two TWNK mutations: overlap of TWNK-related recessive disorders

Exon Sequence of primers Annealing temperature (°C) Amplicon size (bp)
1 (part 1) F: 5′-CTAAGCAGCGAGGAGAGGGGG-3′
R: 5′-TCGCCACGTCCTTCTACAAT-3′
63 543
1 (part 2) F: 5′-GAGGACGACGAGGAGATGCTGTG-3′
R: 5′-CCCATGCCCCCGCAAATAC-3′
63 571
1 (part 3) F: 5′-TGCGTGGGGAGTGGATGG-3′
R: 5′-GCTGGGTCGGGGAATAGTGGT-3′
63 677
1 (part 4) F: 5′-GTGTGCGATATCTGCGACCTG-3′
R: 5′-CATTGTCATGGCCCAAACCC-3′
63 746
2 F: 5′-TTTGGGCCATGACAATGTT-3′
R: 5′-GGAGATGGGGGAGTTCCTAC-3′
60 467
3 F: 5′-CTTCTGCCTGGGGTGGTC-3′
R: 5′-TCCATACATCCTGCTGCCATAC-3′
60 348
4 F: 5′-GTATGGCAGCAGGATGTATGGA-3′
R: 5′-TGTTCGGATGGACAGTCAAGA-3′
60 413
5 F: 5′-CCTCTCCCCATTCTTATCACT-3′
R: 5′-AGCTCAGGACCACAGGATAG-3′
60 518
  1. F forward, R reverse