Table 3 Primers for PCR amplification of TWNK exons and exon–intron boundaries
Exon | Sequence of primers | Annealing temperature (°C) | Amplicon size (bp) |
|---|---|---|---|
1 (part 1) | F: 5′-CTAAGCAGCGAGGAGAGGGGG-3′ R: 5′-TCGCCACGTCCTTCTACAAT-3′ | 63 | 543 |
1 (part 2) | F: 5′-GAGGACGACGAGGAGATGCTGTG-3′ R: 5′-CCCATGCCCCCGCAAATAC-3′ | 63 | 571 |
1 (part 3) | F: 5′-TGCGTGGGGAGTGGATGG-3′ R: 5′-GCTGGGTCGGGGAATAGTGGT-3′ | 63 | 677 |
1 (part 4) | F: 5′-GTGTGCGATATCTGCGACCTG-3′ R: 5′-CATTGTCATGGCCCAAACCC-3′ | 63 | 746 |
2 | F: 5′-TTTGGGCCATGACAATGTT-3′ R: 5′-GGAGATGGGGGAGTTCCTAC-3′ | 60 | 467 |
3 | F: 5′-CTTCTGCCTGGGGTGGTC-3′ R: 5′-TCCATACATCCTGCTGCCATAC-3′ | 60 | 348 |
4 | F: 5′-GTATGGCAGCAGGATGTATGGA-3′ R: 5′-TGTTCGGATGGACAGTCAAGA-3′ | 60 | 413 |
5 | F: 5′-CCTCTCCCCATTCTTATCACT-3′ R: 5′-AGCTCAGGACCACAGGATAG-3′ | 60 | 518 |