Table 1 Primers and probes used for quantification of HSV-1 viral genes
Gene name | Protein name | Primer/probe | Sequence | References |
|---|---|---|---|---|
RL2 | ICP0 | Primer F | 5′-AACTCGTGGGTGCTGATTGAC-3′ | [51] |
Primer R | 5′-CAGGTCTCGGTCGCAGGGAAAC-3′ | |||
Probe | 5′FAM-AGCCCGCCCCGGATGTCTGGG-TAMRA 3′ | |||
UL29 | ICP8 | Primer F | 5′-CACCAGGTTGCGCATCAG-3′ | [53] |
PRIMER R | 5′-CTGCATACGGTGGTGAACAAC-3′ | |||
Probe | 5′FAM-ACCTCGCGGTCCACG-TAMRA-3′ | |||
UL54 | ICP27 | Primer F | 5′-CGCCAAGAAAATTTCATCGAG-3′ | [49] |
Primer R | 5′-ACATCTTGCACCACGCCAG-3′ | |||
Probe | 5′FAM-CTGGCCTCCGCCGACGAGAC-TAMRA 3′ | |||
UL30 | HSV pol | Primer F | 5′-CATCAGCGACCCGGAGAGGGAC-3′ | [50] |
Primer R | 5′-GGGCCAGGCGCTTGTTGGTGTA-3′ | |||
Probe | 5′FAM-CCGCCGAACTGAGCAGACACCCGCGC-TAMRA3′ | |||
UL48 | VP16 | Primer F | 5′-CCGGGTCCGGGATTTACC-3′ | [52] |
Primer R | 5′-CTCGAAGTCGGCCATATCCA-3′ | |||
Probe | 5′FAM-CCCCACGACTCCGCC-TAMRA 3′ | |||
UL41 | UL41 | Primer F | 5′-GGACATCCGCGACGAAAAC-3′ | [52] |
Primer R | 5′-AGAAACCTGTCGGCGATATCAG-3′ | |||
Probe | 5′FAM-CTGGCGCGATCTATC-TAMRA 3′ | |||
LAT | LAT | Primer F | 5′-GCATAGAGAGCCAGGCACAAAA-3′ | [52] |
Primer R | 5′-ACGTACTCCAAGAAGGCATGTG-3′ | |||
Probe | 5′FAM-TCCCACCCCGCCTGTG-TAMRA 3′ |