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Table 1 Primers and probes used for quantification of HSV-1 viral genes

From: Herpes simplex virus-1 (HSV-1) infection induces a potent but ineffective IFN-λ production in immune cells of AD and PD patients

Gene name Protein name Primer/probe Sequence References
RL2 ICP0 Primer F 5′-AACTCGTGGGTGCTGATTGAC-3′ [51]
Primer R 5′-CAGGTCTCGGTCGCAGGGAAAC-3′
Probe 5′FAM-AGCCCGCCCCGGATGTCTGGG-TAMRA 3′
UL29 ICP8 Primer F 5′-CACCAGGTTGCGCATCAG-3′ [53]
PRIMER R 5′-CTGCATACGGTGGTGAACAAC-3′
Probe 5′FAM-ACCTCGCGGTCCACG-TAMRA-3′
UL54 ICP27 Primer F 5′-CGCCAAGAAAATTTCATCGAG-3′ [49]
Primer R 5′-ACATCTTGCACCACGCCAG-3′
Probe 5′FAM-CTGGCCTCCGCCGACGAGAC-TAMRA 3′
UL30 HSV pol Primer F 5′-CATCAGCGACCCGGAGAGGGAC-3′ [50]
Primer R 5′-GGGCCAGGCGCTTGTTGGTGTA-3′
Probe 5′FAM-CCGCCGAACTGAGCAGACACCCGCGC-TAMRA3′
UL48 VP16 Primer F 5′-CCGGGTCCGGGATTTACC-3′ [52]
Primer R 5′-CTCGAAGTCGGCCATATCCA-3′
Probe 5′FAM-CCCCACGACTCCGCC-TAMRA 3′
UL41 UL41 Primer F 5′-GGACATCCGCGACGAAAAC-3′ [52]
Primer R 5′-AGAAACCTGTCGGCGATATCAG-3′
Probe 5′FAM-CTGGCGCGATCTATC-TAMRA 3′
LAT LAT Primer F 5′-GCATAGAGAGCCAGGCACAAAA-3′ [52]
Primer R 5′-ACGTACTCCAAGAAGGCATGTG-3′
Probe 5′FAM-TCCCACCCCGCCTGTG-TAMRA 3′