Fig. 5

Three ES events in STS samples validated via polymerase chain reaction. a–c A schematic diagram of MDM2_22969_ES, MFF_57798_ES, and CD74_ 74077_ES events. The ES events of MDM2, MFF, and CD74 occurred in exons 10 and 11, exon 9, and exon 8, respectively. To validate these events, upstream and downstream primers were designed on either side of the skipping exons. If the ES event did not occur, all the exons would have been retained; electrophoresis produced a single band, and the size of the PCR product was the maximum. Otherwise, the skipping exon was deleted and produced another band. d An electropherogram of the three ES events. M represents a DNA marker; N represents the negative control without a template. Lanes 1 and 2 are products of the PCR amplification of MDM2_22969_ES events in two STS tissues; only a single band of 305 bp is found. Lanes 3 and 4 are products of the PCR amplification of MFF_57798_ES events in two STS tissues, and there are two bands of 324 bp and 105 bp. Lanes 5 and 6 are products of the PCR amplification of CD74_ 74077_ES events in two STS tissues; both 292 bp and 100 bp bands are found. ES Exon Skip, STS soft tissue sarcoma