Fig. 5
AKF-PD inhibited the MAPK and GSK-3β pathways in vivo. a WB analysis of MAPK signaling in rat livers. The p-ERK1/2, p-P38 and p-JNK levels were measured by calculating ratios to the total ERK1/2, total P38 and total JNK levels, respectively. b WB analysis of p-GSK-3β levels in rat livers. GAPDH levels were analyzed as a loading control. n = 3. *P < 0.05 compared with the control group and #P < 0.05 compared with the CCl4 or DMN group