Fig. 3

GSTA3 inhibited HSC activation and fibrogenesis. a WB analysis of α-SMA and FN levels in CFSC-2G and LX2 cells. After an incubation with the GSTA3 siRNA-Lipofectamine 2000 complex for 6 h, HSCs were maintained in culture medium for 48 h before harvest for the protein analysis. b WB analysis of α-SMA and FN levels in CFSC-2G and LX2 cells. After an incubation with the GSTA3 plasmid-Lipofectamine 2000 complex for 6 h, HSCs were maintained in culture medium for 48 h before harvest for the protein analysis. c, d WB analysis of GSTA3, α-SMA and FN levels in CFSC-2G and LX2 cells. After an incubation with the GSTA3 siRNA-Lipofectamine 2000 complex for 6 h, HSCs were maintained in culture medium for 24 h and subsequently treated with 10 ng/ml PDGF-BB for another 24 h before harvest for the protein analysis. e, f WB analysis of GSTA3, α-SMA and FN levels in CFSC-2G and LX2 cells. CFSC cells were incubated with the GSTA3 plasmid-Lipofectamine 2000 complex for 6 h and then maintained in culture medium for 24 h and subsequently treated with 10 ng/ml PDGF-BB for another 24 h before harvest for the protein analysis. LX2 cells were incubated with LV-GSTA3 for 24 h. Afterwards, LX2 were incubated with culture medium for another 24 h and stimulated with 10 ng/ml PDGF-BB for an additional 24 h. GAPDH levels were analyzed as a loading control. All values are presented as the mean ± SD of three independent experiments. *P < 0.05 compared with the Si-Con group, pc-DNA3.1(+) group or LV-Con group; ^#P < 0.05 compared with the Si-Con + PDGF-BB group or pc-DNA3.1(+)+PDGF-BB group. ^$P < 0.05 compared with the Si-rGSTA3 + PDGF-BB. Si-Con, negative control siRNA; Si-rGSTA3, rat GSTA3 siRNA; Si-hGSTA3, human GSTA3 siRNA; pc-DNA3.1(+), negative control pc-DNA3.1(+); pc-DNA3.1(+)-rGSTA3, rat pc-DNA3.1(+)-GSTA3; pc-DNA3.1(+)-hGSTA3, human pc-DNA3.1(+)-GSTA3