Fig. 2

GSTA3 was downregulated in activated HSCs stimulated with PDGF-BB, and AKF-PD upregulated GSTA3 expression. a, b WB analysis of the levels of the α-SMA, FN and GSTA3 proteins in HSCs. GAPDH levels were analyzed as a loading control. c AKF-PD reduced ROS accumulation in HSCs. Fluorescence microscopy images of ROS in HSCs. Scale bar = 50 µm. Flow cytometry was also used to analyze ROS accumulation in HSCs. Values are presented as the mean ± SD of three independent experiments. *P < 0.05 compared with the control group and ^#P < 0.05 compared with the PDGF-BB treatment group. PDGF-BB, platelet-derived growth factor-BB; ROS, reactive oxygen species; NAC, N-acetylcysteine