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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: Administration of losartan preserves cardiomyocyte size and prevents myocardial dysfunction in tail-suspended mice by inhibiting p47phox phosphorylation, NADPH oxidase activation and MuRF1 expression

Fig. 5

Losartan inhibits tail-suspension (TS)-induced NADPH oxidase activation in mouse hearts. Tail-suspended mice were given losartan or vehicle for 28 days. Cell membranes were isolated from mouse hearts and NADPH oxidase activation was determined by measuring p47phox, p67phox and Rac1 in the membrane fractions relative to Na+/K+ ATPase. a Representative western blots from 2 out of 6 different hearts for p47phox, p67phox, Rac1 and Na+/K+ ATPase in cell membranes. bd Quantitation for p67phox (b), p47phox (c) and Rac1 (d) relative to Na+/K+ ATPase. e, f The protein levels of NOX4 and phosphorylated p47 phox were determined in whole heart lysates by western blot analysis. Upper panels are representative western blots from 2 out of 6 different hearts for NOX4, phosphorylated p47phox and GAPDH. Lower panels are quantitation for NOX4 and phosphorylated p47phox relative to GAPDH. Data are mean ± SD, n = 6 in each group. *P < 0.05 vs. Sham + Vehicle and P < 0.05 vs. TS + Vehicle

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