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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Renal temperature reduction progressively favors mitochondrial ROS production over respiration in hypothermic kidney preservation

Fig. 1

Temperature effects on porcine kidneys. a Oxygen consumption [expressed as ΔhPa (ml/min/gr)] in perfused porcine kidneys. The increasing and decreasing curve are expressed. Based on four independent experiments, expressed as mean, error bars represent SEM. b Average of whole kidney oxygen consumption per temperature. Expressed as mean, error bars represent SEM. #Significant (ANOVA with Bonferroni posthoc, p < 0.001). c State three respiration in isolated mitochondria, in response to glutamate and malate with and an ADP generating system. Based on five independent experiments, expressed as mean, error bars represent SEM. #Significant (ANOVA with Bonferroni posthoc, p < 0.001). d Oxygen consumption relative to 37 °C for perfused kidneys and isolated kidney mitochondria. The theoretical Q10 line is plotted (Q10 = 2). e Relative ROS production, measured as mitochondrial H2O2 production at different temperatures in isolated mitochondria from porcine kidneys. N = 4, expressed as percentage of its normothermic control, error bars represent SEM. *p < 0.01, #p < 0.001 (ANOVA with Bonferroni posthoc). f ROS damage, measured as lipid peroxidation in porcine kidneys before and after 16 h of static cold storage in UW at 4 °C. N = 4, expressed as relative to normothermic, error bars represent SEM. *p < 0.01, (Students t test)

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