Fig. 2
Viral DNA in patients’ peripheral blood. DNA was extracted using the Quick-gDNA™ Blood MidiPrep (Zymo Research, CA). The copy number amount of the ACAM2000 gene A56R was quantified by qPCR using PowerUp™ SYBR® Green Master Mix (Thermofisher, CA). Viral DNA was analyzed by qPCR before treatment (bt), and 1 min (1 min), 60 min (60 min), 1 day (1d), 1 week (1 w), 1 month (1 mo), 3 months (3 mo) and 6 months (6 mo) after treatment