Fig. 5From: Adipose afferent reflex is enhanced by TNFα in paraventricular nucleus through NADPH oxidase-dependent ROS generation in obesity-related hypertensive ratsThe effects of R-7050 pretreatment on the NADPH oxiase activity (f), ROS level (e) and the in situ ROS content (a–d) responses to TNFα in the PVN. In situ ROS was determined with a specific fluorogenic probe by the DHE method (n = 3 for each group). The NADPH oxiase activity was detected by the enhanced lucigenin chemiluminescence method and the ROS level were measured by the lucigenin-derived chemiluminescence method (n = 6 for each group). The values are mean ± SD; *p < 0.05 versus Control + Saline + Saline; #p < 0.05 versus OH + Saline + Saline; &p < 0.05 versus OH + Saline + TNFα. PVN paraventricular nucleus, 3V the third ventricleBack to article page