Fig. 6
From: SNORD89 promotes stemness phenotype of ovarian cancer cells by regulating Notch1-c-Myc pathway
Effects of SNORD89 interference on the expression of c-Myc and Notch1 of ovarian cancer cells. a The mRNA expression of c-Myc and Notch1 was detected in OV and OS cells by qRT-PCR. The mRNA expression of the two genes in OV cells was set as 1. b The representative agarose gel electrophoresis photos showed the expression of c-Myc and Notch1 in OV and OS cells after RT-PCR. GAPDH was used an endogenous control. c The mRNA expression of c-Myc and Notch1 was detected in OV cells transfected with SNORD89 OE or NC plasmids at 24 h by qRT-PCR. The mRNA expression of the two genes in OV cells transfected with NC plasmids was set as 1. d The representative agarose gel electrophoresis photos showed the increased expression of c-Myc and Notch1 in OV cells transfected with SNORD89 OE. e The mRNA expression of c-Myc and Notch1 was detected in OS cells transfected with shRNA-1, shRNA-2, and shRNA-NC plasmids at 48 h by qRT-PCR. f The representative agarose gel electrophoresis photos showed the decreased expression of c-Myc and Notch1 in OS cells transfected with SNORD89 shRNA-1 and shRNA-2. g The representative western blot photos showed the increased expression of c-Myc and Notch1 in OV cells transfected with SNORD89 OE. h The representative western blot photos showed the decreased expression of c-Myc and Notch1 in OS cells transfected with SNORD89 shRNA-1 and shRNA-2. β-actin was used an endogenous control