Fig. 8

Neovessels visualization with CD31 and αSMA labelling. CD31 + staining (red) detects both angiogenic capillaries and arterioles. αSMA+ (green) detects arterioles. a Visualization of capillaries and arterioles in the semimembranosus in normal and ischemic legs. b Visualization of capillaries and arterioles in the gastrocnemius in normal and ischemic legs. c Angiogenesis analysis: fluorescence intensity ratio (CD31+ labelling) in the ischemic gastrocnemius in comparison with controlateral muscle. d Arteriogenesis analysis: Fluorescence intensity ratio (αSMA+/CD31+ labelling) in the ischemic gastrocnemius in comparison with controlateral muscle (*comparison of MSCs group versus BMCs group; ^#S-MSCs versus BMCs; ^¥S-MSCs versus MSCs; ^§BMCs versus vehicle; ^ΔMSCs versus vehicle; ^∞S-MSCs versus vehicle)