Skip to main content

Table 2 Results of clinic samples determined by the Luminex assay

From: Novel method for simultaneously detecting HPA and HLA antibodies using Luminex microbeads

Sample Platelet MFI Luminex results MAIPA results
GPIa/IIa (20) GPIba/IX (36) HLA (50) GPIIb/IIIa (66)
S2 G1 83.59 414.37 1399 72.18 Anti-HLA + anti-HPA-2b Anti-HLA + anti-HPA-2b
G2 89.70 468.21 438.5 108.04
G3 52.42 71.95 3341 86.68
G4 46.49 69.51 1442 58.83
S3 G1 71.05 36.19 1390 70.60 Anti-HLA + anti-HPA-5b Anti-HLA + anti-HPA-5b
G2 57.32 68.15 2469 74.08
G3 83.57 43.42 2776.5 111.12
G4 941.90 71.25 1395 71.52
T5 G1 56.06 21.63 45.19 3430.30 Anti-HPA-1a Anti-HPA-1a
G2 59.48 43.65 116.05 1053.89
G3 69.32 53.31 71.13 1318.20
G4 33.28 36.64 45.39 3997.51
S7 G1 1402.07 39.17 109.89 1355.66 Anti-HPA-5a + anti-HPA-1a Anti-HPA-5a + anti-HPA-1a
G2 1410.06 34.86 111.47 1406.88
G3 1114.12 40.45 33.44 1297.48
G4 1498.50 79.59 106.75 1810.53
T8 G1 33.77 41.91 103.64 1347.68 Anti-HLA + anti-HPA-3a Anti-HLA + anti-HPA-3a
G2 52.63 90.44 3675.49 1764.84
G3 52.21 47.67 2065.35 92.82
G4 51.73 37.52 1078.52 1153.10
S9 G1 76.06 79.44 559.08 876.67 Anti-HLA + anti-HPA-3b Anti-HLA + anti-HPA-3b
G2 76.41 69.50 5511.44 107.48
G3 61.12 79.00 1961.17 785.68
G4 69.75 83.96 3080.16 976.73
T10 G1 69.80 23.18 71.49 77.42 Anti-HPA-5b Anti-HPA-5b
G2 74.64 38.32 69.92 44.98
G3 70.63 31.47 69.48 97.10
G4 1979.27 80.06 78.05 54.36
S11 G1 50.80 47.89 28.64 423.74 Anti-HPA-1a Anti-HPA-1a
G2 56.66 71.67 37.95 1022.23
G3 93.93 80.44 64.35 1248.52
G4 43.16 35.45 72.41 1203.06
S12 G1 35.40 22.88 3135.34 91.55 Anti-HLA Anti-HLA
G2 41.92 74.05 14,619.87 130.06
G3 31.82 68.35 11,924.19 85.82
G4 54.81 29.88 9249.19 83.82
Cut-off 132.29 85.19 131.97 163.46 132.29   
  1. The table showed the results of 9 positive clinic samples determined by the Luminex assay. The Platelet represents the platelet donors (G1–G4) reacting with the samples. GPIa/IIa (20), GPIba/IX (36), HLA (50), and GPIIb/IIIa (66) are the monoclonal antibody coupled microbeads, the number in the brackets are the beads code. MFI represents the MFI values of coupled-beads reacted with the responding serum sample-platelet complex measured using a Luminex 100 instrument. Luminex results was inferred from the MFI value and platelet donor genotype; MAIPA was applied previously results. The cut-off MFI value is defined as the mean value of the negative control + 3SD value, and ≥ cut-off value is considered as positive