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Table 1 Expansion of T-cells in the Quantum system

From: Large-scale expansion and characterization of CD3+ T-cells in the Quantum® Cell Expansion System

Donor PBMCs seeded (×106) CD3+ T-cells seeded (%) Total cells harvested (×109) Viability (%) CD3+ T-cells harvested (%) CD3+ T- cell fold-expansion CD3+ T-cell doubling time (h) CD3+ T-cells population doublings Cell culture concentration at harvest (×106 cells/mL)a
1 30 36.9 14.9 91.9 94.2 1268 20.7 10.3 120
2 46.6 13.9 92.5 96.1 956 21.6 9.9 112
3 55.0 16.1 93.8 97.5 951 21.6 9.9 130
4 32.5 16.2 94.0 96.0 1595 20.1 10.6 131
5 23.6 13.1 92.5 96.6 1787 19.8 10.8 106
Mean   38.9 14.8 93.7 96.1 1311 20.7 10.3 120
SD 12.2 1.3 2.0 1.2 376 0.8 0.4 11
% CV 31.4 9.1 2.1 1.3 29 4.0 4.1 9.1
1 85 36.9 27.8 91.0 91.9 815 19.6 9.7 224
2 46.6 24.6 92.0 94.3 586 20.6 9.2 198
3 55.0 26.9 94.0 94.3 543 20.3 9.1 217
4 32.5 23.5 93.0 92.7 789 19.2 9.6 190
5 23.6 22.9 90.3 94.5 1079 18.8 10.1 185
Mean   38.9 25.1 92.1 93.5 762 19.9 9.5 203
SD 12.2 2.1 1.5 1.2 214 0.8 0.4 17
% CV 31.4 8.5 1.6 1.3 28 4.2 4.1 8.5
  1. Fold expansion, doubling times and population doublings were calculated as described in “Materials and methods
  2. aTotal cell concentration at harvest was calculated from the total cells harvested and the volume within the fibers of the bioreactor (124 mL)