Table 1 Expansion of T-cells in the Quantum system
From: Large-scale expansion and characterization of CD3+ T-cells in the Quantum® Cell Expansion System
Donor | PBMCs seeded (×106) | CD3+ T-cells seeded (%) | Total cells harvested (×109) | Viability (%) | CD3+ T-cells harvested (%) | CD3+ T- cell fold-expansion | CD3+ T-cell doubling time (h) | CD3+ T-cells population doublings | Cell culture concentration at harvest (×106 cells/mL)a |
|---|---|---|---|---|---|---|---|---|---|
1 | 30 | 36.9 | 14.9 | 91.9 | 94.2 | 1268 | 20.7 | 10.3 | 120 |
2 | 46.6 | 13.9 | 92.5 | 96.1 | 956 | 21.6 | 9.9 | 112 | |
3 | 55.0 | 16.1 | 93.8 | 97.5 | 951 | 21.6 | 9.9 | 130 | |
4 | 32.5 | 16.2 | 94.0 | 96.0 | 1595 | 20.1 | 10.6 | 131 | |
5 | 23.6 | 13.1 | 92.5 | 96.6 | 1787 | 19.8 | 10.8 | 106 | |
Mean | 38.9 | 14.8 | 93.7 | 96.1 | 1311 | 20.7 | 10.3 | 120 | |
SD | 12.2 | 1.3 | 2.0 | 1.2 | 376 | 0.8 | 0.4 | 11 | |
% CV | 31.4 | 9.1 | 2.1 | 1.3 | 29 | 4.0 | 4.1 | 9.1 | |
1 | 85 | 36.9 | 27.8 | 91.0 | 91.9 | 815 | 19.6 | 9.7 | 224 |
2 | 46.6 | 24.6 | 92.0 | 94.3 | 586 | 20.6 | 9.2 | 198 | |
3 | 55.0 | 26.9 | 94.0 | 94.3 | 543 | 20.3 | 9.1 | 217 | |
4 | 32.5 | 23.5 | 93.0 | 92.7 | 789 | 19.2 | 9.6 | 190 | |
5 | 23.6 | 22.9 | 90.3 | 94.5 | 1079 | 18.8 | 10.1 | 185 | |
Mean | 38.9 | 25.1 | 92.1 | 93.5 | 762 | 19.9 | 9.5 | 203 | |
SD | 12.2 | 2.1 | 1.5 | 1.2 | 214 | 0.8 | 0.4 | 17 | |
% CV | 31.4 | 8.5 | 1.6 | 1.3 | 28 | 4.2 | 4.1 | 8.5 |