Fig. 6

MSCs promoted M2 macrophage polarization and anti-inflammatory effects in high glucose conditions with LPS stimulation. Rat peritoneal macrophages were separated and divided into the normal, HG + LPS and HG + LPS + MSCs groups. After treatment, macrophages from the different groups were collected, and the M1 ratios, M2 ratios and the ratios of M1 to M2 phenotypes were detected by flow cytometry (a). The data were analyzed using Cellquest software (b). The IL-6, TNF-α and IL-10 concentrations in the supernatants were determined by ELISA (c). n = 4 per group. *P < 0.05, **P < 0.01 vs. normal group, ##P < 0.01 vs. HG + LPS group