Fig. 4

iHDAC leads to the expansion of the CD11b^low/Ly6C^low subset upon wound injury. Upon skin injury the inflammatory infiltrate was quantified by flow cytometry. Although the injury mobilized peripheral blood cells between day 1 and day 3 after wound induction to compose the inflammatory infiltrate (a–h), the blockade of HDAC activity did not alter the recruitment dynamics of blood subsets observed in the control group along seven days post injury (i). N = 6 ***p < 0.001. However, when this population was phenotyped for the Ly6C^low and Ly6C^high subsets, we were able to observe a significant increase in the Ly6C^low subset in iHDAC treated skins at 7 days after injury (j, k). N = 4; ***p < 0.001, by the two-way ANOVA test followed by the test of Bonferroni to correct the value of p