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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: The epigenome as a putative target for skin repair: the HDAC inhibitor Trichostatin A modulates myeloid progenitor plasticity and behavior and improves wound healing

Fig. 2

The CD11blow/Ly6Clow subset is the specific target of iHDAC. Analysis after 48 h of culture by flow cytometry of non-adherent bone marrow cells from GM-CSF or GM-CSF + iHDAC treated groups. a, b Immuno positive cells for acetylated histone H4 were observed only in the GM-CSF + iHDAC treated cells (b; b’ DAPI; b” merge). GM-CSF treated cells did not stain for acetylated histone H4 (a; a’ DAPI; a” merge). c, d Acetylated levels of histone H4 were significantly higher in iHDAC treated cells. Phenotypic analysis of myeloid cells through the CD11b and Ly6C showed that the Cd11blow/Ly6Clow harbor significant higher levels of acetylated histone 4 (eg). Representative MFI histogram of P10 (CD11bhigh/Ly6Clow) and P11 (CD11blow/Ly6Clow) subsets shows significant increase of acetylated histone H4 levels in the P11 subset (hl). n = 4 animals per group; Data are mean ± SD. Differences statistically significant, *p < 0.05, **p < 0.01, by the two-way ANOVA followed by the Bonferroni test for correction of the p value

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