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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Intranodal administration of mRNA encoding nucleoprotein provides cross-strain immunity against influenza in mice

Fig. 4

Analysis of systemic responses and immune cell populations in the BALF after H1N1 challenge. BALB/c mice were immunized twice with 17 µg of NP or control mRNA (Ctrl) or 50 µg of HA mRNA with 1 week between immunizations. 8 weeks after boost immunizations, mice (n = 6/group) were infected with 1xLD50 of influenza A H1N1 PR8. On day 6 after challenge, spleens and BALF were isolated. a IFN-γ ELISPOT on splenocytes isolated from vaccinated animals on day 6 after challenge and re-stimulated with the indicated peptides. Images of wells are representative for all mice for the respective conditions. bl Flow cytometric analysis of immune cell populations within the BALF. AM, Alveolar macrophages. b Absolute cell count of CD45+ cells. c Absolute cell count of CD45+ subsets. d Donut-charts showing the composition of CD45+ subsets per group. Fractions show the median of the indicated subpopulations. The number in the center shows the median number of CD45+ cells for that group. ek Percentages of CD45+ subsets within the CD45+ population. l Percentage of MHC II expressing AM within the general AM population. For all graphs, except donut-charts, results are shown as median ± IQR and each symbol represents one mouse for a total of 6 mice per group. Differences between groups were calculated using one-way ANOVA

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