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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: Preparing for cell culture scale-out: establishing parity of bioreactor- and flask-expanded mesenchymal stromal cell cultures

Fig. 5

Immunomodulatory capacity of flask-, spinner- and Scinus-cultured MSC. a, b Induction of CD274 and IDO protein expression in response to pro-inflammatory cytokine IFNγ. Induction is expressed as MFI (stimulated cells)/MFI (unstimulated cells). The Friedman test showed no statistically significant difference in IFNγ-induced upregulation of CD274 and IDO between cells cultured in different culture conditions, χ2(2) = 1.600, p = 0.449 and χ2(2) = 2.333, p = 0.311 respectively. The Kruskal–Wallis H test showed a statistically significant difference in IFNγ-induced upregulation of CD274 and IDO between MSCs grown from the different bone marrow donors, χ2(2) = 11.500, p = 0.021 and χ2(2) = 11.367, p = 0.023, respectively. (a, b inlays) A Pearson correlation analysis confirmed this primary role of donor-related factors in the induction variation by indicating a strong correlation between IFNγ-induced upregulation of CD274 and IDO between Flask- and Scinus-expanded MSCs (R2 = 0.78 and 0.93 respectively). c MSC capacity to inhibit T cell proliferation. d Heatmap and cluster analysis of T cell proliferation

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