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Fig. 8 | Journal of Translational Medicine

Fig. 8

From: A strategy to protect off-the-shelf cell therapy products using virus-specific T-cells engineered to eliminate alloreactive T-cells

Fig. 8

CMVSTs expressing iCHAR do not lower frequency of virus specific T-cells present in allogeneic PBMCs. a Allogeneic PBMCs were either cultured alone or with iCHAR CMVST for 5 days and then co-cultures were pulsed with various viral pepmixes. IFNy staining was performed after overnight incubation with viral pepmixes. Shown are representative dot plots of IFNy expression from gated T-cells in the PBMCs after stimulation with Adenovirus (AdV), CMV or EBV pepmixes. Irrelevant represents pepmix for Survivin which should not elicit a response in PBMCs. b Quantification of IFNy expression from gated T-cells in PBMCs. Values for each virus were calculated by subtracting from Irrelevant control (mean ± SEM, n = 3). Significance was determined by paired two-tailed Student’s t-test. ns not significant

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