Fig. 7

CMVST expressing iCHAR can degranulate when specifically targeted by cognate TCR. a CMVST that were unpulsed or LML pulsed were co-cultured with autologous T-cells expressing sTCR for 4 h and then stained for CD107a. Representative dot plots showing gating of CellTrace Violet + NT CMVSTs (Left) and CD107a expression for NT CMVSTs (Right). b Quantification showing percent of gated NT CMVSTs expressing CD107a (mean ± SEM, n = 3). c Gating for the iCHAR CMVST condition was first done on CellTrace Violet + CMVSTs and then on high iCHAR + T-cells. d Representative dot plots showing CD107a expression from gated iCHAR + CMVSTs. e Quantification showing percent of gated iCHAR + CMVSTs expressing CD107a (mean ± SEM, n = 3). Significance was determined by paired two-tailed Student’s t-test. *p < 0.05. ns not significant