Fig. 1

Characterization of Chimeric HLA Accessory Receptor (CHAR). a Design of CHAR construct in retrovirus backbone. The Bridge spans the distance between B2M and the cell surface. The Q8 marker contains an epitope from CD34. b Schematic of HLA class I molecule (Left) and CHAR (Middle) forming a complex (Right). c Daudi cells were transduced with the CHAR construct and stained for expression of HLA class I molecules using a HLA-ABC antibody on Day 3. d CHAR transduced Daudi cells were stained for HLA-ABC and CD34 to identify the Q8 marker