Fig. 5

The exposition of melanoma cells to BCM or h-Exos induces transcriptomic modifications. a LCP, SK-Mel28 and WM-266 cells were analyzed by dd-PCR to identify potential gene expression variations induced by BCM stimulation. Dot plots refer to representative genes whose levels were influenced by BCM. Levels of MMP-1, CXCR4, CXCR7, PTH-rP and TGF-β were significantly up-regulated in LCP, while remained unchanged in SK-Mel28 and WM-266 cells. b Similar analyses explored the effects of LCP-derived Exos on not-osteotropic SK-Mel28 and WM-266 cells. A significant up-regulation of CXCR7 (> 3-fold increase) occurred in both cell lines, while stimulation of WM-266 cells with a-Exos failed to produce significant difference from baseline values. Results are expressed as fold change from basal mRNA concentration (copy/µl) and represent mean ± SEM. A threshold > 1.5-fold change (bold line) was arbitrarily identified as significant. *p < 0.05; **p < 0.01; ***p < 0.001