Fig. 1

Preparation of a lymph node for OSNA assay and histological examination. Non-adjacent blocks were alternatively used for either the histological examination or the OSNA assay. For histopathological study, serial sections from the same tissue blocks were analysed by H-E and IHC. The two first sections was stained with H-E and the subsequent two using AE1/AE3 and KRT19 mouse monoclonal antibodies. The remaining resected lymph node was analysed by OSNA assay in single homogenized from 50 to 600 mg