Fig. 8
SDS- and Native-PAGE analyses of ozonated whole blood oxy-Hb from five human samples. Gels I, II and III show Hb samples analysed by SDS-PAGE under reducing conditions (in the presence of DTT). Gels IV, V and VI, show Hb samples analysed by SDS-PAGE under non-reducing conditions (in the absence of DTT). Gels VII, VIII and IX show Hb samples analysed by Native-PAGE. Samples A–E include oxy-Hb samples of the non-diabetic individual A and diabetic patients B to E, respectively. Samples in all gels: First lane—Protein marker; Samples numbered 1–4—non-ozonated and ozone treated samples at 15 μg/ml, 35 μg/ml and 55 μg/ml, respectively