Fig. 3
Macrophage phenotype, recruitment and proliferation and MCP-1 secretion in epididymal WAT. a Crown like structures (CLS) density in H&E sections. b qRT-PCR analysis of macrophage marker F4/80 expression in the WAT. c Intracellular staining for Ki67. d MCP-1 ELISA in WAT homogenate. e–l qRT-PCR analysis for gene expression of M1 (e–h) and M2 macrophage markers (i–l) in ATM. Each transcript was normalized to that in CTR + H2O (n = 6 mice/group). Mean ± SEM. n = 5–6 mice/group