Fig. 2

Flow cytometric analysis of epididymal WAT leukocytes and Kupffer cells. a Gating strategy. b–j Immunostaining for markers of T cells (CD3, CD4, and CD8, b–d), ATM and Kupffer cells (CD11b and F4/80, e, h), eosinophils (Siglec-F, f), and neutrophils (Gr1, g). i M1 Kupffer cells are identified by CD80 expression. j M2 Kupffer cells are identified by CD206 expression. Mean ± SEM (n = 10–12 mice/group)