Fig. 1

Fat weight, adipocyte area, serum cholesterol and hepatosteatosis in DIO mice. a Body weight gain over time; b epididymal WAT weight at week 12; c mean adipocyte area. d Representative images of H&E-stained sections of epididymal WAT at ×10; arrows indicate CLS. Each image is representative of 6 mice. e Glucose tolerance test (GTT) and f AUC after 16 h fasting (n = 10). g Total, HDL and LDL/VLDL cholesterol and h ALT activity. Mean ± SEM, n = 10. i H&E staining of liver sections from representative mice from each group with ×10 magnification (upper panel) and with ×20 magnification (lower panel); CV: lobular central vein. Each image is representative of 6 mice. Arrows indicate areas with lipid droplets deposition. AUC, area under the curve. ALT, alanine aminotransferase