Fig. 8

Effect of CD4⁺CD25⁺ T cells from TBM on IFN-γ production. CD4⁺CD25⁺ T cells from TBM either on control or SM16 diet were co-cultured with CD4⁺CD25⁻ responder T cells from naive donors (ratio: 1:1) and stimulated with irradiated APC and soluble anti-CD3 mAb. After 72 h supernatants were harvested from the co-cultures and were analyzed for IFN-γ concentrations. The amounts of IFN-γ production were evaluated in triplicate using OptEIA ELISA kit (Pharmingen). The presented value of IFN-γ production corresponds to 5 × 10⁴ cells. Bars represent mean values ± SEM (n = 3 from a single experiment). Significance between control and experimental groups were determined by Student’s t-test