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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Blockade of TGF-β signaling to enhance the antitumor response is accompanied by dysregulation of the functional activity of CD4+CD25+Foxp3+ and CD4+CD25Foxp3+ T cells

Fig. 4

In vitro suppressive activity of CD4+CD25+FoxP3+ (a) and CD4+CD25FoxP3+ (b) T cells isolated from naive or SM16 treated FoxP3eGFP mice. CD4+CD25+FoxP3+ and CD4+CD25FoxP3+ cells from donor mice were obtained by FACS sorting. The activity of isolated cells having the ability to suppress naive T cell proliferation in an in vitro assay was tested by adding increasing numbers of CD4+CD25+FoxP3+ or CD4+CD25FoxP3+ T cells to co-cultures of sorted CD4+CD25 (responder cells) and APCs from naive mice in the presence of anti-CD3. After 48 h the plates were pulsed for 18 h with [3H]-thymidine. Subsequently, the cells were harvested on glass fiber filters and assessed for uptake of the labeled thymidine by liquid scintillation (4 and 3 independent experiments respectively). Repeat assays of CD4+CD25+Foxp3+ cells were performed using a single batch of naive responder mice. Similarly, repeat assays of CD4+ CD25Foxp3+ cells were performed using a separate batch of responder cells from naive mice

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