Fig. 3

Gal1 induced HOMEC proliferation via the ERK1/2 and PI3K pathways, but not AKT pathway. Cells were seeded in 2% gelatin pre-coated 96 well plates at a density of 10,000 cells/well in starvation media containing 2% FCS. After overnight incubation, cells were treated ± Gal1 (50 ng/ml) and in the absence or presence of a U0126 (10 µmol/l) and PD98059 (25 µmol/L), b LY294002 (25 µmol/l) and MK2206 (5 µmol/l) and incubated for 72 h. Commercially available WST-1 assay was used to assess cellular proliferation. Results are mean ± SD and shown as percentage of the control, ***p < 0.001 vs control (100%), ###p < 0.001 vs Gal1 (normalised to control 100%), n = 14–15. n.s. denotes not significant