Fig. 3

a Flow cytometry viability assay. APOCC and OVCAR3 were treated or not with 100 nM cisplatin and 4 nM taxol alone or in presence of E4⁺ECs for 48 h. Viability was assessed by flow cytometry using Vioblue. b Notch expression quantification. APOCC and OVCAR3 were treated or not with 100 nM cisplatin and 4 nM taxol. Surviving APOCC and OVCAR3 were sorted and Notch3 expression was quantified by flow cytometry. c, d Viability assay. OCCs in coculture with E4⁺ECs were treated either with 100 nM cisplatin and 4 nM taxol, or with 100 nM cisplatin, 4 nM taxol and GSI; Control: OCCs cocultured with E4⁺ECs in DMSO. Cells were stained with live/dead and live cells (in red) were quantified by flow cytometry. The histogram displays the percentage of living cells for each condition. e Viability assay. OVCAR3 were treated with 100 nM cisplatin and 4 nM taxol after treatment with human recombinant (rh) Jagged1 or not. Viability was assessed by flow cytometry. f Phosphokinase array. OVCAR3 were treated in the presence or not of rh Jagged 1. The significant protein modifications of the phosphokinase array were cut from the full membrane and represent on the left panel. The right panel represents the relative quantification of the dot pixel density. g Phosphokinase array. OVCAR3 were cultured with or without E4⁺ECs and sorted. The significant protein modifications of the phosphokinase array were cut from the full membrane and represent on the left panel. The right panel represents the relative quantification of the dot pixel density. h Viability assay. OVCAR3 were treated with 100 nM cisplatin and 4 nM taxol after treatment with human recombinant (rh) Jagged1 or not. Surviving OVCAR3 were sorted. i Apoptosis array. Surviving OVCAR3 treated with 100 nM cisplatin and 4 nM taxol after upstream treatment with Jagged1 were analyzed using an apoptosis array. The histograms represent the relative quantification of the dot pixel density