Fig. 5
Over-expression of miR-195-5p represses angiogenesis and chemotherapy resistance in OC via the regulation of PSAT1 and the GSK3β/β-catenin signaling pathway. a, b Expression of HIF-1α and VEGF in SKOV-3 cells when transfected or treated with miR-195-5p mimic, oe-PSAT1 and WAY262611 (inhibitor of GSK3β/β-catenin signaling pathway); *p < 0.05 vs. the si-NC group; c effects of different DDP concentrations on the inhibition rate of SKOV-3 cells when treated with DDP, miR-195-5p mimic, oe-PSAT1 and WAY262611 detected by CCK-8; *p < 0.05 vs. the miR-195-5p mimic + oe-NC group, #p < 0.05 vs. the miR-195-5p mimic + DMSO group; d IC50 value to DDP in SKOV-3 cells after treated with DDP, miR-195-5p mimic, oe-PSAT1 and WAY262611 detected by CCK-8; *p < 0.05 vs. the miR-195-5p mimic + oe-NC group; #p < 0.05 vs. the miR-195-5p mimic + DMSO + DDP group; e, f the apoptosis of SKOV-3 cells when treated with DDP, miR-195-5p mimic, oe-PSAT1 and WAY262611 measured by flow cytometry; *p < 0.05 vs. the miR-195-5p mimic + oe-NC + DDP group; #p < 0.05 vs. the miR-195-5p mimic + DMSO + DDP group. The figure data are measurement data and expressed as mean ± standard deviation; one-way ANOVA was used among multiple groups, while repeated measures ANOVA was performed at different time points; the experiment was independently repeated three times