Fig. 7

PI3K/Akt signaling pathway may be involved in the regulation of TIM-1 in the cellular functions of NSCLC cells. The Agilent microarray analysis was used to identify the DEGs between the LV-TIM-1-shRNA group and LV-NC group in A549 and SK-MES-1 cells. a, b The co-up-regulated gene profile. c, d The co-down-regulated gene profile. Then the expressions of PTEN, phos-AKT and total AKT at the protein level were examined by using Western blotting analysis (e). f, g Demonstrated that the expression of PTEN at the protein level was increased, and the expression of phos-AKT at the protein level was decreased after depletion of TIM-1 in both two cell lines, while no significant change was found in the expression of total AKT at the protein level (h)