Fig. 6

Knockdown of CBX3 inhibits cell growth and leads to G2/M cell cycle arrest in U373 cells. a qPCR analysis of CBX3 mRNA levels in SF268, U373, U251, U87MG, U118, and A172 cells. qPCR (b) and Western blot (c) validation of CBX3 expression in U373 cells transfected with shCBX3-1, shCBX3-2, and shCtrl. d CCK8 proliferation curve of U373 cells transfected with shCBX3-1, shCBX3-2, and shCtrl. e The cell cycle was analyzed by flow cytometry in U373 cells transfected with shCtrl, shCBX3-1, and shCBX3-2. **Indicates a p-value of < 0.01; *indicates a p-value of < 0.05