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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: Plasma therapy leads to an increase in functional IgA and IgM concentration in the blood and saliva of a patient with X-linked agammaglobulinemia

Fig. 3

Saliva IgA increases bacterial agglutination. Saliva IgG (a) and IgA (b) was determined by ELISA (n = 4, mean ± standard error of the mean). The arrow indicates IgGRT that was given together with the FFP treatment. NTHi strain 3655 was incubated with patient saliva for 30 min and binding of IgG (c) and IgA (d) were determined by flow cytometry. Measurement of geometric mean fluorescence intensity (MFI) was depicted in arbitrary units (AU) (n = 5, mean ± standard error of the mean). Correlation between binding of saliva IgG (e) or IgA (f) to the bacterial surface and saliva IgG or IgA levels was determined by linear regression, respectively. Bacterial agglutination (g) was measured by flow cytometry (n = 5, mean ± standard error of the mean). Correlation between binding of saliva IgG (h) and IgA (i) to the bacterial surface and percentage agglutination was determined by linear regression

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