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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Plasma therapy leads to an increase in functional IgA and IgM concentration in the blood and saliva of a patient with X-linked agammaglobulinemia

Fig. 2

Serum IgM increases complement binding and bacterial killing. NTHi strain 3655 was incubated with 10% heat inactivated patient’s serum for 30 min and binding of IgG (a) and IgM (b) was determined by flow cytometry. Measurement of geometric mean fluorescence intensity (MFI) was depicted in arbitrary units (AU) (n = 4, mean ± standard error of the mean). The arrow indicates IgGRT that was given together with the FFP treatment. NTHi strain 3655 was incubated with 10% HI patient’s serum and 5% agammaglobulinemia patient’s serum as complement source for 30 min and binding of complement C3 (c) and complement complex C5b9 (d) were determined by flow cytometry. Measurement of geometric mean fluorescence intensity (MFI) was depicted in arbitrary units (AU) (n = 4, mean ± standard error of the mean). Correlation between binding of C3 and IgG (e) or IgM (f) was determined by linear regression. Correlation between binding of C5b9 and IgG (g) or IgM (h) was determined by linear regression. Bacterial survival in 10% HI day 0 pre- and post-FFP serum supplemented with 5% agammaglobulinemia patient’s serum as complement source was determined (n = 5). Bacterial survival is depicted in percentage compared to 5% HI agammaglobulinemia patient’s serum as complement source (n = 5, mean ± standard error of the mean). A two-tailed paired t test was used for statistical analysis. *P < 0.05 (i)

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