Table 1 Summary of all immunization regimens showing the times of immunization (with viruses, IFUs and injections sites used), bleeding to obtain serum samples to assess antibody responses and intracellular cytokine staining (ICS) for the analysis of T-cell responses
From: Virus-like vaccines against HIV/SIV synergize with a subdominant antigen T cell vaccine
Experiment | Group | Time | Intervention | Virus | IFU/mouse | Injection site |
|---|---|---|---|---|---|---|
Immunogenicity of Ad-HIVB | – | Day 0 | Immunization | Ad-HIVB | 1 × 108 | Lower right leg |
Day 18 | ICS | |||||
Immunogenicity of Ad-Ii-SIVCErvv | – | Day 0 | Immunization | Ad-Ii-SIVCErvv | 2 × 108 | Lower right leg |
Day 14 | ICS | |||||
Heterologous HIV-prime SIV-boost | without Ad-Ii-SIVCErvv | Day 0 | Prime immunization | Ad-HIVB | 1 × 108 | Lower right leg |
Day 50–57 | Bleeding | |||||
Day 63–74 | Boost immunization | MVA-SIV | 5 × 107 | Lower right leg | ||
10 days post boost | Bleeding & ICS | |||||
with Ad-Ii-SIVCErvv | Day 0 | Prime immunization | Ad-HIVB | 1 × 108 | Lower right leg | |
Ad-Ii-SIVCErvv | 1 × 108 | Lower left leg | ||||
Day 50–57 | Bleeding | |||||
Day 63–74 | Boost immunization | MVA-SIV | 5 × 107 | Lower right leg | ||
10 days post boost | Bleeding & ICS | |||||
Homologous SIV-prime SIV-boost | without Ad-Ii-SIVCErvv | Day 0 | Prime immunization | Ad-SIV | 2 × 108 | Upper right leg |
Day 56 | Bleeding & | |||||
Boost immunization | MVA-SIV | 1 × 107 | Upper left leg | |||
Day 311 | Bleeding & ICS | |||||
with Ad-Ii-SIVCErvv | Day 0 | Prime immunization | Ad-SIV | 2 × 108 | Upper right leg | |
Ad-Ii-SIVCErvv | 2 × 108 | Upper left leg | ||||
Day 56 | Bleeding & | |||||
Boost immunization | MVA-SIV | 1 × 107 | Upper left leg | |||
Day 311 | Bleeding & ICS |