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Table 1 Summary of all immunization regimens showing the times of immunization (with viruses, IFUs and injections sites used), bleeding to obtain serum samples to assess antibody responses and intracellular cytokine staining (ICS) for the analysis of T-cell responses

From: Virus-like vaccines against HIV/SIV synergize with a subdominant antigen T cell vaccine

Experiment Group Time Intervention Virus IFU/mouse Injection site
Immunogenicity of Ad-HIVB Day 0 Immunization Ad-HIVB 1 × 108 Lower right leg
Day 18 ICS    
Immunogenicity of Ad-Ii-SIVCErvv Day 0 Immunization Ad-Ii-SIVCErvv 2 × 108 Lower right leg
Day 14 ICS    
Heterologous HIV-prime SIV-boost without Ad-Ii-SIVCErvv Day 0 Prime immunization Ad-HIVB 1 × 108 Lower right leg
Day 50–57 Bleeding    
Day 63–74 Boost immunization MVA-SIV 5 × 107 Lower right leg
10 days post boost Bleeding & ICS    
with Ad-Ii-SIVCErvv Day 0 Prime immunization Ad-HIVB 1 × 108 Lower right leg
Ad-Ii-SIVCErvv 1 × 108 Lower left leg
Day 50–57 Bleeding    
Day 63–74 Boost immunization MVA-SIV 5 × 107 Lower right leg
10 days post boost Bleeding & ICS    
Homologous SIV-prime SIV-boost without Ad-Ii-SIVCErvv Day 0 Prime immunization Ad-SIV 2 × 108 Upper right leg
Day 56 Bleeding &    
Boost immunization MVA-SIV 1 × 107 Upper left leg
Day 311 Bleeding & ICS    
with Ad-Ii-SIVCErvv Day 0 Prime immunization Ad-SIV 2 × 108 Upper right leg
Ad-Ii-SIVCErvv 2 × 108 Upper left leg
Day 56 Bleeding &    
Boost immunization MVA-SIV 1 × 107 Upper left leg
Day 311 Bleeding & ICS