Fig. 5

Addition of Ad-Ii-SIVCErvv to homologous SIV-prime SIV-boost regimen did not impact Env-specific antibody responses. Serum samples were taken at the time of the intracellular cytokine staining and analyzed for antibody binding to lyzed pseudoviruses carrying SIVmac239 Env by ELISA. a shows the antibody responses as titers and b as the AUC. In c the avidity index is depicted and in d the antibody responses detected with anti-IgG isotype-specific antibodies as the ratio to the not isotype-specific antibody (pan-IgG). Horizontal lines indicate the geometric mean, except in c where it depicts the mean because of a single mouse not having a measurable avidity index