Fig. 4

CD8+ and CD4+ T-cell responses induced by homologous SIV-prime SIV-boost regimen. CD1 mice were immunized with the priming vectors Ad-SIV (n = 9) or Ad-SIV and Ad-Ii-SIVCErvv (n = 10) and boosted with MVA-SIV 8 weeks after the prime. 10 days later CD8+ and CD4+ T-cell responses were measured by stimulating splenocytes with the noted SIVmac239 peptide pools and analyzing them by intracellular cytokine staining followed by flow cytometry. a, c show the total numbers of IFNγ+ CD8+ (a) and CD4+ (b) T-cells per spleen and b, d the percentage of IFNγ+ TNFα+ CD8+/CD4+ T-cells of IFNγ+ CD8+/CD4+ T-cells for samples with more than 10⁴ IFNγ+ CD8+ T-cells. Horizontal lines mark the geometric mean and significant differences are indicated by asterisks with *(p ≤ 0.05), **(p ≤ 0.01) and ***(p ≤ 0.001)