Fig. 5

In vitro characterization of miRNAs. PC3 prostate carcinoma cells were transfected with control, miR-20a or miR-20b mimics. a miR-20a overexpression increased the number of viable cells (p = 0.03, paired Student’s t test). miR-20b showed no significant differences in cell viability (p = 0.5915, paired Student’s t test). b Soft Agar assay evaluating colony formation in a 3D matrix showed increased colony formation with miR-20a mimic (p = 0.03, paired Student’s t test) and miR-20b mimic (p = 0.01, paired Student’s t test). miR-106a overexpression also increased colony formation, however this did not reach statistical significance (p = 0.11, paired Student’s t test). c Radiation protection factor (RPF) is shown for each miRNA compared to respective control