Fig. 5

The effect of combined AIBP and APOA-I on cholesterol efflux and raft-related signaling. a Cholesterol efflux assay for the indicated cells as described in the Materials and Methods. b HCT116 cells were incubated with APOA-I (10 µg/ml) for 6 h in the presence or absence of AIBP (5, 10 and 20 µg/ml) and cholesterol efflux was measured. c The indicated cells were stained for lipid rafts with cholera toxin B (CTxB, green). Scale bar, 10 μm. d The percentage of cells with high, middle and low lipid raft contents per frame (mean ± SD, n = 10, *P < 0.05; **P < 0.01). e The indicated cells with or without AIBP + APOA-I overexpression, or HCT116 cells treated with or without 10 mM MβCD for 30 min; the cell lysates were separated into Triton X-100 soluble and insoluble fractions as described in the Materials and Methods and blotted with integrin β1, paxillin, vinculin, FAK, Flot-1 and GAPDH. f The indicated cells were treated the same as in (f) and blotted with MT1-MMP, CAV-1, Flot-1 and GAPDH