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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: ALS blood expression profiling identifies new biomarkers, patient subgroups, and evidence for neutrophilia and hypoxia

Fig. 3

Cell types associated with ALS-increased and ALS-decreased DEGs. a Enrichment statistics for 12 cell types (ALS-increased DEGs). b Neutrophil GSEA analysis (ALS-increased DEGs). c RBC lineage GSEA analysis (ALS-increased DEGs). d Enrichment statistics for 12 cell types (ALS-decreased DEGs). e RBC lineage GSEA analysis (ALS-decreased DEGs). f Monocyte GSEA analysis (ALS-decreased DEGs). In a, d, positive statistics indicate over-representation of cell type-specific genes among ALS DEGs (P < 0.05, red bars), and negative statistics indicate under-representation of cell type-specific genes among ALS DEGs (P < 0.05, blue bars). In b, c, e and f, genes are ranked according to their expression in the indicated cell type (horizontal axis), and cumulative abundance of ALS DEGs is shown (vertical axis). The area (lower-right) between the cumulative abundance curve and diagonal is equal to enrichment statistics shown in parts A and D (p-values: Wilcoxon rank sum test). g Cell type assignments (ALS-increased DEGs). h Cell type assignments (ALS-decreased DEGs). In g, h, genes were assigned to the cell type for which they were most highly expressed as compared to other cell types. Pie charts denote the proportion of genes assigned to each cell type (*P < 0.05; **FDR < 0.05, Fisher’s exact test). Genes were not assigned to any cell type if expression was not detectable in at least 10% of samples for any cell type (P < 0.05, Signed rank test). i Top-ranked ALS-increased DEGs and their expression across 12 cell types. j Top-ranked ALS-decreased DEGs and their expression across 12 cell types. In i and j, magenta up-triangles denote the cell type with highest expression for each gene

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