Table 1 Probes distribution on pathogen chip
Probe group type | Number of targets | Number of probes | Purpose |
|---|---|---|---|
All spot | 1010 | 14,716 | RNA pathogens coverage and internal controls |
Pathogen specific (not replicated) | 16 | 1769 | Probes intensity analysis of pathogen specific genes |
Internal control (replicated 10 times) | 902 | 902 | Agilent requirement for probes normalization |
ERCC probes (replicated 45 times) | 84 | 84 | Determination of intra-probe variance |
Negative control (not replicated) | 3 | 120 | Determination of probes cross reactivity |