Fig. 1

Pathogen chip design, plasma samples preparation workflow and analysis strategy. a Sequence accessions for all RNA viruses were retrieved from the NCBI genome sequence databases and candidate as sequences to target. Regions of target sequence unique to the accession were used to select multiple 60-mer probes for microarray synthesis. b A novel workflow design combining two different applications generating cDNA instead of amplified RNA, followed by Cy3 labelling and hybridization based on a DNA application. c Experimental criteria to assess quality of signals generated by probes for each species