Fig. 1

a Treatment with Minnelide at a dose of 0.15 mg/kg and 0.1 mg/kg significantly decreased leukemic burden in cancer bearing mice. b The total burden of disease was calculated using bioluminescence with the In Vivo Imaging System (IVIS) measuring total flux from a region of interest (ROI) drawn over the entire animal. Total flux is calculated as the radiance (photons/s) in each pixel summed or integrated over the ROI area (cm²) × 4π. c AML blasts derived from patient leukepheresis was implanted into NRGS mice after sub lethal irradiation and treatment with Minnelide was started at a dose of 0.1 mg/kg/day after confirming engraftment via flow cytometry. Antibody against human CD45 was used to quantify disease burden. Treatment with Minnelide slowed the progression of AML and decreased the tumor burden at endpoint. d Even when Minnelide treatment was discontinued at 100 days, there was an improvement in median survival of mice from 71 to 150 days. n = 4–5, *p < 0.05 when compared to saline treated animals, data shows mean ± SEM