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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: SOX9 drives the epithelial–mesenchymal transition in non-small-cell lung cancer through the Wnt/β-catenin pathway

Fig. 2

SOX9 promotes NSCLC cell invasion and migration. a SOX9-overexpression cell lines were analyzed by western blotting for SOX9 expression. β-tubulin was used as a loading control. b SOX9-knockdown cell lines were analyzed by western blotting for SOX9 expression. β-tubulin was used as a loading control. c, e Invasion assay with the Trans-well chamber and Matrigel layer was performed with the SOX9-overexpression and SOX9-knockdown cell lines. The number of cells that passed through the bottom of the Trans-well chamber with the Matrigel layer was counted, as shown in g, *p < 0.05. d, f Migration assay with the Trans-well chamber without the Matrigel layer was performed on the SOX9-overexpression and SOX9-knockdown cell lines. The number of cells that passed through the bottom of the Trans-well chamber without the Matrigel layer was counted as shown in h, *p < 0.05. i, j Wound healing assay was employed to detect the cell migration ability of SOX9-overexpressing and SOX9-knockdown cells. k, l Quantify the wound healing assay from i and j, ***p < 0.0001

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