Fig. 2
Role of CEACAM1 in HBE cells with IFN-γ treatment. mRNA expression of IL-6, IL-8, TGFβ, and VEGF in HBE cells treated with vehicle (control) or with IFN-γ at 10 ng/ml for 24 h (a). mRNA expression of IL6 (b) and IL8 (c) in cellCEACAM1− or cellCEACAM1+ treated with vehicle (control) or with IFN-γ at 1.0 or 10 ng/ml. Cell movement measured by wound healing assay (d) and cell proliferation measured by CCK8 0, 12, 24, 48, or 72 h after treatment with vehicle (HBE cell) and treated with IFN-γ. Healing percentage (%) = (S0 − St)/S0 × 100% (S0: wound area at 0 h; St: wound area at specific time point). Cell proliferation rate = (ODt-OD0)/OD0 (OD0: OD value at 450 nm at 0 h after treated with IFN-γ; ODt: OD value at 450 nm at t hours after treated with IFN-γ). *, **, ***p-values less than 0.05, 0.01 and 0.005, as compared to control